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Structure of carbohydrate chain of a thrombin-like protease from the venom of Agkistrodon halys brevicaudus stejneger snake
https://doi.org/10.15012/00000409
https://doi.org/10.15012/000004091df26eb9-e1f1-4ffd-b0a2-2543afcb08ad
名前 / ファイル | ライセンス | アクション |
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jinbun_vol4601_03 (233.6 kB)
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Copyright (c) 2009 SakaiJunichi, RojnuckarinPonlapat, JangprasertPanchalee, NakanoTakahiro, SaitouKenji, YamamotoChikashi, MuraseYutaka, OkadaTadashi
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2015-09-04 | |||||
タイトル | ||||||
タイトル | Structure of carbohydrate chain of a thrombin-like protease from the venom of Agkistrodon halys brevicaudus stejneger snake | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Structure of carbohydrate chain of a thrombin-like protease from the venom of Agkistrodon halys brevicaudus stejneger snake | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | snake venom | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | thrombin-like protease | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | carbohydrate | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ID登録 | ||||||
ID登録 | 10.15012/00000409 | |||||
ID登録タイプ | JaLC | |||||
著者 |
Sakai, Junichi
× Sakai, Junichi× Rojnuckarin, Ponlapat× Jangprasert, Panchalee× Nakano, Takahiro× Saitou, Kenji× Yamamoto, Chikashi× Murase, Yutaka× Okada, Tadashi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The structure of the carbohydrate chain of kangshuanmei, a thrombin-like serine protease isolated from Agkistrodon halys brevicaudus stejneger snake venom, was determined. The carbohydrate content of the kangshuanmei was 18%. The sugar composition was analyzed by the acid hydrolysis followed by aminobenzoic ethyl ester labeling. Galactose, N-acetylglucosamin, mannose, and fucose were detected, indicating that the binding carbohydrate chain is asparagine-linked type oligosaccharides. N-Acetylneuraminic acid located at non-reduced terminal of the carbohydrate chain was identified by neuraminidase digestion. The carbohydrate chain moiety was separated from kangshuanmei by hydrazynolysis treatment followed by aminobenzoic octyl ester (ABOE) labeling. The isolated ABOEmodified carbohydrate chain was compared to the asparagine-linked type standard oligosaccharides. The carbohydrate chains were consisted of sialylated bi(39.4%)-, tri(50.4%)- and tetra(10.2%)- antennary lactosamins complex containing fucose. The structure of the conjugated carbohydrate chain of kangshuanmei was significantly different from that of thrombin, which has a bisected antennary structure of oligosaccharide. | |||||
書誌情報 |
名古屋学院大学論集 人文・自然科学篇 en : THE NAGOYA GAKUIN DAIGAKU RONSHU; Journal of Nagoya Gakuin University; HUMANITIES and NATURAL SCIENCES 巻 46, 号 1, p. 35-43, 発行日 2009-07-31 |
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出版者 | ||||||
出版者 | 名古屋学院大学総合研究所 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0385-0056 |