{"created":"2023-06-19T09:36:58.493569+00:00","id":398,"links":{},"metadata":{"_buckets":{"deposit":"83af39f2-9ef2-43e1-8293-d4e0cdc4b1bf"},"_deposit":{"created_by":2,"id":"398","owners":[2],"pid":{"revision_id":0,"type":"depid","value":"398"},"status":"published"},"_oai":{"id":"oai:ngu.repo.nii.ac.jp:00000398","sets":["1:12:80"]},"author_link":["2165","44","46","622","45","40","623","624"],"item_10002_alternative_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"ヘビドクユライトロンビンヨウセリンコウソニケツゴウスルトウサノヤクワリ"}]},"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-01-31","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"50","bibliographicPageStart":"47","bibliographicVolumeNumber":"47","bibliographic_titles":[{"bibliographic_title":"名古屋学院大学論集　人文・自然科学篇"},{"bibliographic_title":"THE NAGOYA GAKUIN DAIGAKU RONSHU; Journal of Nagoya Gakuin University; HUMANITIES and NATURAL SCIENCES","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Recently, we determined the primary structure of a thrombin-like protease isolated from the venom of Agkistrodon halys brevicaudus stejneger snake. The protease was found to be a single chain glycoprotein with a molecular weight of 32 kDa, containing 18% carbohydrates. The carbohydrate chain binding positions, Asn81, 99 and 148, were located in the neighbourhood of an active site cleft. In this study, the enzymatic property of the deglycosylated protease was compared to the intact protease in order to elucidate the role of the carbohydrate chain. The deglycosylation of the protease was carried out by glycosidase treatment and the deglycosylated protease with a molecular weight of 26.5 kDa was obtained. The intact protease converted human fibrinogen to fibrin. Fibrinopeptide A, B and Bβ1-42 were released during fibrin clot formation. The deglycosylated protease also formed the fibrin clot. Fibrinopeptide A primarily released although the release of the fibrinopeptide B and Bβ1-42 was significantly reduced. Removal of the carbohydrate moiety resulted in a decrease in these fragment releases, suggesting that the access of the fibrinogen Bβ chain to the active site was restricted by the deglycosylation. Thus, the carbohydrate chain may play an important role in the conformational integrity of the active site and also in the interaction between the protease and substrates, especially a large molecular substrate such as fibrinogen.","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15012/00000390","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"名古屋学院大学総合研究所"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0385-0056","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"酒井, 淳一"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"中野, 貴博"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"齋藤, 健治"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山本, 親"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"村瀬, 豊"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"岡田, 忠"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"箭頭, 真理子"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"竹田, 忠紘"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2015-09-04"}],"displaytype":"detail","filename":"jinbun_vol4702_05.pdf","filesize":[{"value":"306.2 kB"}],"format":"application/pdf","license_note":"Copyright (c) 2011 酒井淳一, 中野貴博, 齋藤健治, 山本親, 村瀬豊, 岡田忠, 箭頭真理子, 竹田忠紘","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"jinbun_vol4702_05","url":"https://ngu.repo.nii.ac.jp/record/398/files/jinbun_vol4702_05.pdf"},"version_id":"dc7e750c-dfe8-4b05-a02a-a8996eaea41c"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"snake venom","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"thrombin-like serine protease","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"carbohydrate chain","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"蛇毒由来トロンビン様セリン酵素に結合する糖鎖の役割","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"蛇毒由来トロンビン様セリン酵素に結合する糖鎖の役割"},{"subitem_title":"Role of carbohydrate chain of a thrombin-like protease isolated from the venom of Agkistrodon halys brevicaudus stejneger snake","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"2","path":["80"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-09-04"},"publish_date":"2015-09-04","publish_status":"0","recid":"398","relation_version_is_last":true,"title":["蛇毒由来トロンビン様セリン酵素に結合する糖鎖の役割"],"weko_creator_id":"2","weko_shared_id":2},"updated":"2023-06-19T10:03:06.027633+00:00"}